Journal: Infection and Drug Resistance

Article Title: Next-generation-sequencing technology used for the detection of Mycoplasma hominis in renal cyst fluid: a case report

doi: 10.2147/IDR.S198678

Figure Lengend Snippet: The coverage profile and phylogenetic tree of the M. hominis strain X.

Article Snippet: The anti-infective drug sensitivity test was done with M. hominis IST (BioMerieux, France), which is a widely applied commercial Mycoplasma culture, detecting and drug sensitivity test kit with satisfying sensitivity and specificity , .

Techniques:

Journal: Infection and Drug Resistance

Article Title: Next-generation-sequencing technology used for the detection of Mycoplasma hominis in renal cyst fluid: a case report

doi: 10.2147/IDR.S198678

Figure Lengend Snippet: NGS result

Article Snippet: The anti-infective drug sensitivity test was done with M. hominis IST (BioMerieux, France), which is a widely applied commercial Mycoplasma culture, detecting and drug sensitivity test kit with satisfying sensitivity and specificity , .

Techniques: Bacteria

Journal: European Journal of Clinical Microbiology & Infectious Diseases

Article Title: MYCO WELL D-ONE detection of Ureaplasma spp. and Mycoplasma hominis in sexual health patients in Wales

doi: 10.1007/s10096-020-03993-7

Figure Lengend Snippet: Sensitivity, specificity, positive predictive value, negative predictive value and accuracy for the MYCO WELL D-ONE detection of Mycoplasma hominis

Article Snippet: The culture titration assay for Ureaplasma has been previously described [ ] and was modified to include the additional titration of M . hominis in parallel using M . hominis- specific medium (MHSM) purchased from CPM SAS (Rome, Italy).

Techniques:

Journal: European Journal of Clinical Microbiology & Infectious Diseases

Article Title: MYCO WELL D-ONE detection of Ureaplasma spp. and Mycoplasma hominis in sexual health patients in Wales

doi: 10.1007/s10096-020-03993-7

Figure Lengend Snippet: Flow chart displaying CLSI-compliant AST of M . hominis presumptively identified as resistant by MYCO WELL D-ONE. The MYCO WELL D-ONE over-reported clindamycin resistance ( p = 0.05; Fisher’s exact test). One further tetracycline-resistant M . hominis isolate was found although it was missed by the initial assay screening

Article Snippet: The culture titration assay for Ureaplasma has been previously described [ ] and was modified to include the additional titration of M . hominis in parallel using M . hominis- specific medium (MHSM) purchased from CPM SAS (Rome, Italy).

Techniques:

Journal: European Journal of Clinical Microbiology & Infectious Diseases

Article Title: MYCO WELL D-ONE detection of Ureaplasma spp. and Mycoplasma hominis in sexual health patients in Wales

doi: 10.1007/s10096-020-03993-7

Figure Lengend Snippet: Nucleotide alignment of the tet (M) gene found in the U . parvum and M . hominis strains, both co-incidentally isolated from the same patient. Shown here are nineteen of the twenty-four mismatches in nucleotide identity were clustered in a 180 nucleotide stretch in the middle of the 1920 nucleotide open reading frame, confirming different origins for these genes and not transfer between these two co-infecting species

Article Snippet: The culture titration assay for Ureaplasma has been previously described [ ] and was modified to include the additional titration of M . hominis in parallel using M . hominis- specific medium (MHSM) purchased from CPM SAS (Rome, Italy).

Techniques: Isolation

Journal: European Journal of Clinical Microbiology & Infectious Diseases

Article Title: MYCO WELL D-ONE detection of Ureaplasma spp. and Mycoplasma hominis in sexual health patients in Wales

doi: 10.1007/s10096-020-03993-7

Figure Lengend Snippet: Bacterial loads for Ureaplasma spp. ( a ) or M . hominis ( b ) in swab or urine samples. Bacterial loads from urine samples were more frequently amenable for AST, based on CLSI guidelines, for < 10 5 CCU/ml (black fraction of the bar). a For Ureaplasma -positive urine samples 5.9% possessed a load > 10 5 CCU/mL (grey fraction of the bar), whereas for Ureaplasma -positive swab samples, 55.9% possessed a load > 10 5 CCU/mL. The prevalence of a Ureaplasma load of > 10 5 CCU/mL captured on swab samples was significantly higher than that of urine samples, for Ureaplasma positive patients ( p ≤ 0.0001; Fisher’s exact test). b For M . hominis -positive urine samples, 7% possessed a load > 10 5 CCU/mL. Whereas for M . hominis -positive swab samples, 40% possessed a load > 10 5 CCU/mL. The prevalence of a M . hominis load of > 10 5 CCU/mL captured on swab samples was significantly higher than that of urine samples, for M . hominis positive patients ( p = 0.0004; Fisher’s exact test)

Article Snippet: The culture titration assay for Ureaplasma has been previously described [ ] and was modified to include the additional titration of M . hominis in parallel using M . hominis- specific medium (MHSM) purchased from CPM SAS (Rome, Italy).

Techniques:

Journal: European Journal of Clinical Microbiology & Infectious Diseases

Article Title: MYCO WELL D-ONE detection of Ureaplasma spp. and Mycoplasma hominis in sexual health patients in Wales

doi: 10.1007/s10096-020-03993-7

Figure Lengend Snippet: Bacterial load comparison for matched Ureaplasma -positive ( a ) or M. hominis -positive ( b ) patient samples. a All paired Ureaplasma -positive samples possessed bacterial loads equal to or greater their urine counterparts. Statistical analysis revealed swabs to possess significantly greater Ureaplasma loads than urines ( p = 0.0255; paired t test). b 76.2% of paired M . hominis -positive samples possessed bacterial loads equal to or greater their urine counterparts. However, 2 urine samples detected M . hominis where swab samples did not and 2 swab samples detected M . hominis in patients with negative culture results from urine. A universal trend of lower bacterial load in urine compared to matched swabs was not always seen for this organism. Statistical analysis of matched M . hominis -positive samples failed to achieve significance ( p = 0.0939; paired t test)

Article Snippet: The culture titration assay for Ureaplasma has been previously described [ ] and was modified to include the additional titration of M . hominis in parallel using M . hominis- specific medium (MHSM) purchased from CPM SAS (Rome, Italy).

Techniques: Comparison

Journal: European Journal of Clinical Microbiology & Infectious Diseases

Article Title: MYCO WELL D-ONE detection of Ureaplasma spp. and Mycoplasma hominis in sexual health patients in Wales

doi: 10.1007/s10096-020-03993-7

Figure Lengend Snippet: Bar and whisker graphs displaying the min-max detection times for Ureaplasma spp. and M . hominis . Samples from males and females are examined separately comparing the MYCO WELL D-ONE assay to culture titration assay in specialist media. Mean values are represented by + symbol. Time to detection of Ureaplasma spp. in female samples was 28.14 h (culture titration) and 27.57 (MYCO WELL D-ONE) ( a ) and 34.09 h in males was for both assays ( b ). Time to detection of M . hominis in females was 43.26 and 43 h for MYCO WELL D-ONE and culture titration, respectively ( c ), and 46.25 h in male samples ( d ). There was no significant difference in time to detection between assays

Article Snippet: The culture titration assay for Ureaplasma has been previously described [ ] and was modified to include the additional titration of M . hominis in parallel using M . hominis- specific medium (MHSM) purchased from CPM SAS (Rome, Italy).

Techniques: Whisker Assay, Titration

Journal: European Journal of Clinical Microbiology & Infectious Diseases

Article Title: MYCO WELL D-ONE detection of Ureaplasma spp. and Mycoplasma hominis in sexual health patients in Wales

doi: 10.1007/s10096-020-03993-7

Figure Lengend Snippet: Details of resistant isolates

Article Snippet: The culture titration assay for Ureaplasma has been previously described [ ] and was modified to include the additional titration of M . hominis in parallel using M . hominis- specific medium (MHSM) purchased from CPM SAS (Rome, Italy).

Techniques: Mutagenesis